1 sample(s) found by the keyword GSM98599.


  1. GEO sample ID: GSM98599
    • Sample_geo_accession: GSM98599
    • Sample_status: Public on May 30 2006
    • Sample_submission_date: Feb 28 2006
    • Sample_last_update_date: Mar 19 2007
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: Saccharomyces cerevisiae cells
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Saccharomyces cerevisiae, wt, maintianed on YPD plates, started from 48 hrs growth on a new YPD agar plate
    • Sample_treatment_protocol_ch1: Cells were grown to O.D. of 1.0, given 10?g/ml Azinomycin B in minimal ethanol, and grown for 6 hours, then collected by centrifugation, frozen in liquid nitrogen, thawed before proecessing.
    • Sample_growth_protocol_ch1: Cells were grown in YPD media at 30 degrees Celsius at 250 rpm shaker.
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: SDS/double phenol RNA extraction of total RNA was performed according to the manufacturer's instructions.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
    • Sample_hyb_protocol: Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip YeastS98 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
    • Sample_scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
    • Sample_description: We have examined azinomycin B against cultures of Saccharomyces cerevisiae and observed cytotoxic activity at moderate concentrations (ED50
    • Sample_data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Coran,MH,Watanabe
    • Sample_contact_email: watanabe@mail.chem.tamu.edu
    • Sample_contact_phone: 979-458-8094
    • Sample_contact_fax: 979-458-8095
    • Sample_contact_laboratory: Watanabe
    • Sample_contact_department: Chemistry
    • Sample_contact_institute: Texas A*M University
    • Sample_contact_address: Texas A&M Dept. Chemistry MS 3255
    • Sample_contact_city: College Station
    • Sample_contact_state: TX
    • Sample_contact_zip/postal_code: 77843
    • Sample_contact_country: USA
    • Sample_supplementary_file: NONE
    • Sample_series_id: GSE4311
    • Sample_data_row_count: 9335
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: nab3 ts mutant at 37C
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 0.3 3 A
    AFFX-MurIL10_at 0.4 1.3 A
    AFFX-MurIL4_at 1 5.3 A
    AFFX-MurFAS_at 2.3 8.6 A
    AFFX-BioB-5_at 230.6 92.5 P
    AFFX-BioB-M_at 271.1 140.4 P
    AFFX-BioB-3_at 206.1 83.4 P
    AFFX-BioC-5_at 325.8 207.9 P
    AFFX-BioC-3_at 321.3 202.8 P
    AFFX-BioDn-5_at 387.9 266.9 P
    View Full Table






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