1 sample(s) found by the keyword GSM6715.

  1. GEO sample ID: GSM6715
    • Sample_geo_accession: GSM6715
    • Sample_status: Public on May 30 2003
    • Sample_submission_date: May 23 2003
    • Sample_last_update_date: Oct 28 2005
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: Total RNA
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_molecule_ch1: total RNA
    • Sample_description: An overnight culture of parental strain wild type (BY4741) grown in YPD was diluted 50-fold in fresh YPD, grown on an Orbital shaker for 2 or 3 hours, then glucosamine was added to a final concentration of 15 mM. Cells were grown in glucosamine for 2 hours to an OD600 of 1.6-1.8 and harvested by centrifugation. Total RNA was isolated by a hot phenol procedure (Schmitt et al. 1990, Nucleic Acids Research 18: 3091-3092). Target probe preparation was done according to Affymetrix Gene Expression Technical Manual (Affymetrix, Santa Clara CA). Briefly, first strand and double-stranded cDNA were synthesized from total RNA (10-40 ug) (SuperScript II RT). cDNA was converted to biotinylated complementary RNA by in vitro transcription containing T7 RNA polymerase (Enzo Biochem). The cRNA product was cleaned up on RNeasy spin columns (Qiagen) followed by quantitation spectrophotometrically (UV lambda=260 nm) and by gel electrophoresis. About 20 ug of cRNA was fragmented to yield 35-200 bases fragments before hybridization. RNA probes were hybridized to the entire yeast genome microarray (YG-S98, Affymetrix) for 16 hours at 45 C. After washing in a non-stringent and stringent wash buffer, the probe arrays were stained with streptavidin phycoerythrin in the GeneChip Fluidics Station and scanned with the Affymetrix GeneChip Scanner according to manufacturer’s instructions. Gene chips were analyzed and normalized by GeneChip Analysis Suite. Three replicates labeled #1, #2, and #3 were performed.
    • Sample_description: Keywords
    • Sample_platform_id: GPL90
    • Sample_contact_name: Garrett,M,Frampton
    • Sample_contact_email: frampton@mit.edu
    • Sample_contact_laboratory: Richard Young
    • Sample_contact_department: Biology
    • Sample_contact_institute: Whitehead Instute - MIT
    • Sample_contact_address: 9 Cambridge Center
    • Sample_contact_city: Cambridge
    • Sample_contact_state: MA
    • Sample_contact_zip/postal_code: 02142
    • Sample_contact_country: USA
    • Sample_supplementary_file: NONE
    • Sample_series_id: GSE441
    • Sample_data_row_count: 9335
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: WT (BY4741)+GlcN (2hr) #2
    ID_REF Corrected Value VALUE ABS_CALL
    10000_at 20.7 11.8 A
    10001_at 685.1 663.9 P
    10002_i_at 2964.6 2948.5 A
    10003_f_at 8409.3 8408.6 P
    10004_at 1.1 7.3 A
    10005_at 224.9 314 P
    10006_at 2.2 89.1 P
    10007_at 688.2 802.5 P
    10008_at 0.1 4.4 A
    10009_at 0.3 8.6 A
    View Full Table

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