1 sample(s) found by the keyword GSM6713.


  1. GEO sample ID: GSM6713
    • Sample_geo_accession: GSM6713
    • Sample_status: Public on May 30 2003
    • Sample_submission_date: May 23 2003
    • Sample_last_update_date: Oct 28 2005
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: Total RNA
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_molecule_ch1: total RNA
    • Sample_description: An overnight culture of parental wild type strain (BY4741) grown in YPD was diluted 50-fold in fresh YPD, grown further to an OD_600 of 1.6-1.8 and harvested by centrifugation. Total RNA was isolated by a hot phenol procedure (Schmitt et al. 1990, Nucleic Acids Research 18: 3091-3092). Target probe preparation was done according to Affymetrix Gene Expression Technical Manual (Affymetrix, Santa Clara CA). Briefly, first strand and double-stranded cDNA were synthesized from total RNA (10-40 ug) (SuperScript II RT). cDNA was converted to biotinylated complementary RNA by in vitro transcription containing T7 RNA polymerase (Enzo Biochem). The cRNA product was cleaned up on RNeasy spin columns (Qiagen) followed by quantitation spectrophotometrically (UV lambda
    • Sample_description: Keywords
    • Sample_platform_id: GPL90
    • Sample_contact_name: Garrett,M,Frampton
    • Sample_contact_email: frampton@mit.edu
    • Sample_contact_laboratory: Richard Young
    • Sample_contact_department: Biology
    • Sample_contact_institute: Whitehead Instute - MIT
    • Sample_contact_address: 9 Cambridge Center
    • Sample_contact_city: Cambridge
    • Sample_contact_state: MA
    • Sample_contact_zip/postal_code: 02142
    • Sample_contact_country: USA
    • Sample_supplementary_file: NONE
    • Sample_series_id: GSE441
    • Sample_data_row_count: 9335
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: WT (BY4741)+GlcN (2hr) #1
    ID_REF Corrected Value VALUE ABS_CALL
    10000_at 34 14.9 P
    10001_at 500.3 470 P
    10002_i_at 2295.2 2282.4 A
    10003_f_at 8427 8427 P
    10004_at 1.6 3.8 A
    10005_at 293.7 366 P
    10006_at 15.1 123.5 P
    10007_at 374.6 484.4 P
    10008_at 0.3 5.2 A
    10009_at 0.9 42.9 A
    View Full Table






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