1 sample(s) found by the keyword GSM60933.


  1. GEO sample ID: GSM60933
    • Sample_geo_accession: GSM60933
    • Sample_status: Public on Jun 15 2006
    • Sample_submission_date: Jun 14 2005
    • Sample_last_update_date: Jun 16 2005
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: strain BY4743, grown in shaker
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: BY4743 (MAT a/alpha his3D1, leu2D, met15D ura3D)
    • Sample_biomaterial_provider_ch1: Invitrogen
    • Sample_growth_protocol_ch1: Culture was grown for 18 hours at 30 C in a rotating wall vessel placed in a gyrorotatory shaker at 90 rpm
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Total RNA was extracted using a combination of the hot phenol/glass beads method of Ausubel et al. (Ausubel FM, Brent E, Kingstion RE, Moore DD, Seidman JG, Smith JA, and Struhl K. Current Protocols in Molecular Biology, New York: Wiley, 1995) and TRIZOL (Sigma).
    • Sample_label_ch1: Biotin
    • Sample_label_protocol_ch1: Total RNA (10 µg) was converted into double-stranded cDNA (ds-cDNA) by using SuperScript Choice System (Life Technologies, Rockville, MD) with an oligo(dT) primer containing a T7 RNA polymerase promoter (Genset, San Diego, CA). After second-strand synthesis, the reaction mixture was extracted with phenol-chloroform-isoamyl alcohol, and ds-cDNA was recovered by ethanol precipitation.
    • Sample_hyb_protocol: Standard Affymetrix procedures
    • Sample_scan_protocol: Standard Affymetrix procedures
    • Sample_description: Saccharomyces cerevisiae strain BY4743 gorwn in a rotating wall vessel (RWV) for 18 hours in a gyrorotatory shaker
    • Sample_data_processing: Affymetrix Microarray Suite version 5.0
    • Sample_platform_id: GPL90
    • Sample_contact_name: Timothy,,Hammond
    • Sample_contact_email: thammond@tulane.edu
    • Sample_contact_department: Department of Medicine, Nephrology Section, SL-45
    • Sample_contact_institute: Tulane University Health Sciences Center
    • Sample_contact_address: 1430 Tulane Ave
    • Sample_contact_city: New Orleans
    • Sample_contact_state: LA
    • Sample_contact_zip/postal_code: 70115
    • Sample_contact_country: USA
    • Sample_supplementary_file: NONE
    • Sample_series_id: GSE2818
    • Sample_data_row_count: 9335
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: BY4743_rotator_rep1
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 0.2 1.6 A
    AFFX-MurIL10_at 0.7 0.8 A
    AFFX-MurIL4_at 5.5 1.1 A
    AFFX-MurFAS_at 10.9 1 A
    AFFX-BioB-5_at 102 60.4 P
    AFFX-BioB-M_at 98 62.5 P
    AFFX-BioB-3_at 100 67.2 P
    AFFX-BioC-5_at 141.8 107.4 P
    AFFX-BioC-3_at 130.7 92.9 P
    AFFX-BioDn-5_at 101.4 63.2 P
    View Full Table






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