1 sample(s) found by the keyword GSM37623.


  1. GEO sample ID: GSM37623
    • Sample_geo_accession: GSM37623
    • Sample_status: Public on Dec 17 2004
    • Sample_submission_date: Dec 15 2004
    • Sample_last_update_date: Oct 28 2005
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: chemostat sampled cells
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_molecule_ch1: total RNA
    • Sample_description: The S. cerevisiae strains used in this study was the haploid, prototrophic strain CEN.PK113-7D (MATa LEU3). Strains were grown at 30 °C in 2-liter chemostats (Applikon), with a working volume of 1.0 liter as described in van den Berg et al.. Cultures were fed with defined synthetic media based on the medium described by Verduyn et al . For nitrogen-limited growth the medium contained the following: (NH4)2SO4, 1.0 g·l-1; K2SO4, 5.3 g·l-1; KH2PO4, 3.0 g·l-1; MgSO4·7H2O, 0.5 g·l-1; vitamins and trace elements. Glucose concentrations in the feed were designed to obtain approximately 17 g·l-1 residual glucose in the culture broth and thus to avoid transcriptional responses as a result of different degrees of glucose repression. Feed concentrations were 59 g·l-1 glucose (reference strain) and 53 g·l-1 glucose (leu3D strain). The dilution rate was set at 0.10 h-1. The pH was measured online and kept constant at 5.0 by the automatic addition of 2 M KOH with the use of an Applikon ADI 1030 biocontroller. Stirrer speed was 800 rpm and the airflow was 0.5 l.min-1. Dissolved oxygen tension was measured online with an Ingold model 34 100 3002 probe, and was above 50% air saturation. A condenser connected to a cryostat set at 2 °C cooled the off-gas, and oxygen and carbon dioxide were measured off-line. Steady-state samples were taken between 10 and 14 volume changes to avoid strain adaptation due to long term cultivation. Dry weight, metabolite-, and gas profiles were constant over at least 3 volume changes prior to sampling for RNA extraction
    • Sample_platform_id: GPL90
    • Sample_contact_name: Jean-Marc,,Daran
    • Sample_contact_email: j.g.daran@tudelft.nl
    • Sample_contact_phone: +31 15 278 2412
    • Sample_contact_fax: +31 15 278 23 55
    • Sample_contact_laboratory: Kluyver centre for genomics of industrial organisms
    • Sample_contact_department: Department of Biotechnology
    • Sample_contact_institute: Delft University of Technology
    • Sample_contact_address: Julianalaan 67
    • Sample_contact_city: Delft
    • Sample_contact_zip/postal_code: 2628BC
    • Sample_contact_country: Netherlands
    • Sample_supplementary_file: NONE
    • Sample_series_id: GSE2076
    • Sample_data_row_count: 9335
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Wild type (first experiment)
    ID_REF Corrected Value VALUE ABS_CALL
    10000_at 1.7 4.6 A
    10001_at 104.9 104.6 P
    10002_i_at 562.8 561.8 A
    10003_f_at 1590 1583.1 P
    10004_at 0.1 1.7 A
    10005_at 120.1 143.8 P
    10006_at 6.3 50.3 P
    10007_at 90.9 126 P
    10008_at 0 2.8 A
    10009_at 0.1 11.9 P
    View Full Table






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