1 sample(s) found by the keyword GSM346651.


  1. GEO sample ID: GSM346651
    • Sample_geo_accession: GSM346651
    • Sample_status: Public on Dec 04 2008
    • Sample_submission_date: Nov 29 2008
    • Sample_last_update_date: Dec 03 2008
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: hst3hst4 mutant
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Strain: YCB600 (FY background)
    • Sample_characteristics_ch1: HST3 and HST4 genes, which encode putative NAD-dependent deacetylases that regulate histone 3 K56 acetylation, are deleted.
    • Sample_biomaterial_provider_ch1: Jef Boeke laboratory at the Johns Hopkins University School of Medicine.
    • Sample_growth_protocol_ch1: Saccharomyces cerevisiae were grown in YPD media.
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Hot acid phenol extraction following purification using RNeasy kit (Qiagen).
    • Sample_label_ch1: Biotin
    • Sample_label_protocol_ch1: One round amplification protocol for total RNA following Affymetrix’ specifications using T7 promoter oligo dT primer for cDNA synthesis, and ENZO IVT kit.
    • Sample_hyb_protocol: 16hrs at 45°C with rotation (60rpm).
    • Sample_scan_protocol: Using Affymetrix’ GeneChip Scanner and default parameters described by the manufacturer.
    • Sample_description: Hybridization was performed by the Johns Hopkins Medical Institutions Microarray Core Facility.
    • Sample_data_processing: Image analysis using GCOS 1.4, using MAS 5.0 algorithm and manufacturer’s specifications. Global scaling of images to a target intensity of 150.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Ivana,,Celic
    • Sample_contact_email: icelic@jhmi.edu
    • Sample_contact_institute: JHSOM
    • Sample_contact_address: 725 N Wolfe Street
    • Sample_contact_city: Baltimore
    • Sample_contact_zip/postal_code: 21211
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM346nnn/GSM346651/GSM346651.CEL.gz
    • Sample_series_id: GSE13766
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: hst3hst4_2a
    ID_REF Corrected Value VALUE
    AFFX-MurIL2_at 0.4 1.5
    AFFX-MurIL10_at 1.9 0.7
    AFFX-MurIL4_at 10.6 3.9
    AFFX-MurFAS_at 16.9 2.8
    AFFX-BioB-5_at 213.6 168.9
    AFFX-BioB-M_at 273.6 233.1
    AFFX-BioB-3_at 205.2 171.5
    AFFX-BioC-5_at 364.6 322.3
    AFFX-BioC-3_at 358.7 313
    AFFX-BioDn-5_at 381.2 344.6
    View Full Table






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