1 sample(s) found by the keyword GSM304531.


  1. GEO sample ID: GSM304531
    • Sample_geo_accession: GSM304531
    • Sample_status: Public on Oct 27 2008
    • Sample_submission_date: Jul 09 2008
    • Sample_last_update_date: Oct 24 2008
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: Snf1-I132G+2NMPP1_Raffinose_2
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: PY133 (Mat a his3del200 ura3-52 snf1del) transformed with HA-SNF1-I132G allele
    • Sample_biomaterial_provider_ch1: Karen Arndt
    • Sample_treatment_protocol_ch1: 25 micromolar 2NM-PP1 for 40 min
    • Sample_growth_protocol_ch1: grown in SC-ura medium+2% glucose to early log phase and then transferred to 2% raffinose + 0.05% glucose for 120 min. before adding 25 micromolar 2NM-PP1 for 40 min
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Total RNA prepared by glass bead lysis, digested with DNase and purified by RNeasy Kit (Qiagen). Sample quality assessed by BioAnalyzer (Agilent).
    • Sample_label_ch1: Biotin
    • Sample_label_protocol_ch1: per Manufacturer's instructions-BioArray High Yield RNA Transcript Labeling Kit (Enzo)
    • Sample_hyb_protocol: Affymetrix GeneChip Fluidics Station 450
    • Sample_scan_protocol: Affymetrix GeneChip Fluidics Station 450 and DAT files generated by Microarray Analysis Suite 5.0 (MAS5)
    • Sample_description: 3_110805.mas5.CHP
    • Sample_data_processing: MAS 5.0; scaled to target of 500; default setting used.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Karen,M. ,Arndt
    • Sample_contact_email: arndt@pitt.edu
    • Sample_contact_fax: 412 624 4759
    • Sample_contact_laboratory: Karen Arndt
    • Sample_contact_department: Biological Sciences
    • Sample_contact_institute: University of Pittsburgh
    • Sample_contact_address: 4249 Fifth Avenue
    • Sample_contact_city: Pittsburgh
    • Sample_contact_state: PA
    • Sample_contact_zip/postal_code: 15260
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM304nnn/GSM304531/GSM304531.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM304nnn/GSM304531/GSM304531.CHP.gz
    • Sample_series_id: GSE12061
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Snf1+2NMPP1_Raffinose_2
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 1.03136 3.24928 A
    AFFX-MurIL10_at 3.17349 3.49744 A
    AFFX-MurIL4_at 9.87256 4.30754 A
    AFFX-MurFAS_at 18.5584 1.0685 A
    AFFX-BioB-5_at 374.47 227.328 P
    AFFX-BioB-M_at 604.219 465.57 P
    AFFX-BioB-3_at 487.372 357.241 P
    AFFX-BioC-5_at 766.23 635.651 P
    AFFX-BioC-3_at 871.389 749.953 P
    AFFX-BioDn-5_at 1420.66 1305.86 P
    View Full Table






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