1 sample(s) found by the keyword GSM304520.


  1. GEO sample ID: GSM304520
    • Sample_geo_accession: GSM304520
    • Sample_status: Public on Oct 27 2008
    • Sample_submission_date: Jul 09 2008
    • Sample_last_update_date: Oct 24 2008
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: Snf1-I132G+2NMPP1_Raffinose_1
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: PY133 (Mat a his3del200 ura3-52 snf1del) transformed with HA-SNF1-I132G allele
    • Sample_biomaterial_provider_ch1: Karen Arndt
    • Sample_treatment_protocol_ch1: 25 micromolar 2NM-PP1 for 40 min
    • Sample_growth_protocol_ch1: grown in SC-ura medium+2% glucose to early log phase and then transferred to 2% raffinose + 0.05% glucose for 120 min. before adding 25 micromolar 2NM-PP1 for 40 min
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Total RNA prepared by glass bead lysis, digested with DNase and purified by RNeasy Kit (Qiagen). Sample quality assessed by BioAnalyzer (Agilent).
    • Sample_label_ch1: Biotin
    • Sample_label_protocol_ch1: per Manufacturer's instructions-BioArray High Yield RNA Transcript Labeling Kit (Enzo)
    • Sample_hyb_protocol: Affymetrix GeneChip Fluidics Station 450
    • Sample_scan_protocol: Affymetrix GeneChip Fluidics Station 450 and DAT files generated by Microarray Analysis Suite 5.0 (MAS5)
    • Sample_description: 3_042006.mas5.CHP
    • Sample_data_processing: MAS 5.0; scaled to target of 500; default setting used.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Karen,M. ,Arndt
    • Sample_contact_email: arndt@pitt.edu
    • Sample_contact_fax: 412 624 4759
    • Sample_contact_laboratory: Karen Arndt
    • Sample_contact_department: Biological Sciences
    • Sample_contact_institute: University of Pittsburgh
    • Sample_contact_address: 4249 Fifth Avenue
    • Sample_contact_city: Pittsburgh
    • Sample_contact_state: PA
    • Sample_contact_zip/postal_code: 15260
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM304nnn/GSM304520/GSM304520.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM304nnn/GSM304520/GSM304520.CHP.gz
    • Sample_series_id: GSE12061
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Snf1+2NMPP1_Raffinose_1
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 1.03802 23.2183 A
    AFFX-MurIL10_at 2.2823 0.950256 A
    AFFX-MurIL4_at 15.7023 8.7974 A
    AFFX-MurFAS_at 31.2263 3.06044 A
    AFFX-BioB-5_at 407.817 248.514 P
    AFFX-BioB-M_at 607.102 454.111 P
    AFFX-BioB-3_at 501.874 357.857 P
    AFFX-BioC-5_at 699.697 566.879 P
    AFFX-BioC-3_at 909.151 760.927 P
    AFFX-BioDn-5_at 1318.7 1190.41 P
    View Full Table






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