1 sample(s) found by the keyword GSM299775.


  1. GEO sample ID: GSM299775
    • Sample_geo_accession: GSM299775
    • Sample_status: Public on Jun 01 2009
    • Sample_submission_date: Jun 24 2008
    • Sample_last_update_date: Jun 25 2008
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: University Of Cardiff, Simon Reed lab
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: BY4742 MAT alpha hisdelta1leu2delta0 lys2delta0 ura3delta0 YER162c::his3 YEL037c::kanMX4
    • Sample_biomaterial_provider_ch1: Euroscarf
    • Sample_treatment_protocol_ch1: untreated
    • Sample_growth_protocol_ch1: Grow cells in YPD till they reach OD 0.5-0.6
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Trizol Total RNA purification protocol can be found http://www.cogeme.man.ac.uk/services/transcriptoneservicefacility/index.asp
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Affymetrix Genechip Expression Amplification one-cycle target labeling reagents
    • Sample_hyb_protocol: Eukaryotic Target preparation Manual
    • Sample_scan_protocol: Eukaryotic Target preparation Manual
    • Sample_description: roarray experiments were performed using a yeast S98 chip oligonucleotide array (Affymetrix, Inc.) according to the manufacturer's instructions (http://www.affymetrix.com/support/technical/manuals.affx). Approximately 1 µg of total RNA was processed into biotinylated cRNA according to Affymetrix protocols; 15-µg biotinylated cRNA targets were fragmented and hybridized to the arrays at 45°C for 16 h. The arrays were then processed using Affymetrix fluidics protocol EukGE-WS2 (V4 450) and stained with R-phycoerythrin conjugated to streptavidin (Molecular Probes, Inc). Microarray images were acquired using the 2500 GeneChip scanner (Affymetrix) and Microarray Suite version 5.0 software. Robust multichip average normalization and further analysis were carried out using the Affymetrix library of procedures (Affy version 1.5.8) in Bioconductor (version 1.5) (http://www.bioconductor.org) within R (version 2.0.1) (http://www.r-project.com; 16).
    • Sample_data_processing: RMA
    • Sample_platform_id: GPL90
    • Sample_contact_name: Julia,,Smirnova
    • Sample_contact_email: smirnovaj@cardiff.ac.uk
    • Sample_contact_department: Cardiff School of Medicine
    • Sample_contact_institute: University of Cardiff
    • Sample_contact_address: Tenovus building Ground Floor
    • Sample_contact_city: Cardiff
    • Sample_contact_zip/postal_code: CF14 4XN
    • Sample_contact_country: UK
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM299nnn/GSM299775/GSM299775.CEL.gz
    • Sample_series_id: GSE11871
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: rad4delta rad23delta strain R2
    ID_REF Corrected Value VALUE
    10000_at 3.77 4.01
    10001_at 6.55 6.56
    10002_i_at 9 9
    10003_f_at 11.75 11.8
    10004_at 2.77 2.83
    10005_at 8.2 8.29
    10006_at 5.69 5.88
    10007_at 8.41 8.61
    10008_at 3.39 3.79
    10009_at 5.3 5.53
    View Full Table






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