1 sample(s) found by the keyword GSM296157.


  1. GEO sample ID: GSM296157
    • Sample_geo_accession: GSM296157
    • Sample_status: Public on Feb 05 2009
    • Sample_submission_date: Jun 03 2008
    • Sample_last_update_date: Feb 05 2009
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: 30C anaerobic N-lim chemostat
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Characteristics S. cerevisiae prototrophic haploid reference strain CEN.PK113-7D (MATa)
    • Sample_biomaterial_provider_ch1: JM Daran
    • Sample_treatment_protocol_ch1: Culture quenched in liquid nitrogen
    • Sample_growth_protocol_ch1: The S. cerevisiae prototrophic haploid reference strain CEN.PK113-7D (MATa) was grown at a dilution rate (D) of 0.03 h-1 at 30oC in 2-liter chemostats (Applikon, Schiedam, The Netherlands) with a working volume of 1.0 liter as described in {van den Berg, 1996;Tai, 2005}. A temperature probe connected to a cryostat controlled cultures grown at 30oC. Cultures were grown in a defined synthetic medium that was limited by nitrogen with all other growth requirements in excess as described by {Tai, 2005}. The dilution rate was set at 0.03 hr-1 with pH measured online and kept constant at 5.0 by automatic addition of 2 M KOH using an Applikon ADI 1030 Biocontroller. The stirrer speed was set to 600 rpm. Anaerobic growth and steady state conditions were maintained as described {Tai, 2005}. Biomass dry weight, metabolite, dissolved oxygen, and gas profiles were constant at least three volume changes prior to sampling.
    • Sample_growth_protocol_ch1: Tai, S. L., Boer, V. M., Daran-Lapujade, P., Walsh, M. C., de Winde, J. H., Daran, J. M. & Pronk, J. T. (2005) J. Biol. Chem. 280, 437-447.
    • Sample_growth_protocol_ch1: van den Berg, M. A., de Jong-Gubbels, P., Kortland, C. J., van Dijken, J. P., Pronk, J. T. & Steensma, H. Y. (1996) J. Biol. Chem. 271, 28953-28959.
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Sampling of cells from chemostats, probe preparation, and hybridization to Affymetrix Genechip┬« microarrays were performed as recommended by Affymetrix
    • Sample_label_ch1: Biotinylated dUTP - streptavidine Phycoerythrin (SAPE)
    • Sample_label_protocol_ch1: Sampling of cells from chemostats, probe preparation, and hybridization to Affymetrix Genechip┬« microarrays were performed as recommended by Affymetrix
    • Sample_hyb_protocol: Sampling of cells from chemostats, probe preparation, and hybridization to Affymetrix Genechip┬« microarrays were performed as recommended by Affymetrix
    • Sample_scan_protocol: Affymetrix scanner 3000
    • Sample_description: ES54
    • Sample_data_processing: GCOS V 1.2
    • Sample_platform_id: GPL90
    • Sample_contact_name: Jean-Marc,,Daran
    • Sample_contact_email: j.g.daran@tudelft.nl
    • Sample_contact_phone: +31 15 278 2412
    • Sample_contact_fax: +31 15 278 23 55
    • Sample_contact_laboratory: Kluyver centre for genomics of industrial organisms
    • Sample_contact_department: Department of Biotechnology
    • Sample_contact_institute: Delft University of Technology
    • Sample_contact_address: Julianalaan 67
    • Sample_contact_city: Delft
    • Sample_contact_zip/postal_code: 2628BC
    • Sample_contact_country: Netherlands
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM296nnn/GSM296157/GSM296157.CEL.gz
    • Sample_series_id: GSE11452
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Anaerobic glucose limited chemostat
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 1.5 1.1 A
    AFFX-MurIL10_at 5.3 1.4 A
    AFFX-MurIL4_at 9.8 2.3 A
    AFFX-MurFAS_at 11.9 1.8 A
    AFFX-BioB-5_at 79.6 37.8 P
    AFFX-BioB-M_at 88.9 48.5 P
    AFFX-BioB-3_at 83.0 44.3 P
    AFFX-BioC-5_at 124.6 83.9 P
    AFFX-BioC-3_at 140.5 97.8 P
    AFFX-BioDn-5_at 242.0 200.2 P
    View Full Table






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