1 sample(s) found by the keyword GSM283695.


  1. GEO sample ID: GSM283695
    • Sample_geo_accession: GSM283695
    • Sample_status: Public on Feb 05 2009
    • Sample_submission_date: Apr 23 2008
    • Sample_last_update_date: Feb 05 2009
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: Chemostat sample
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Haploid prototrophic Saccharomyces cerevisiae CEN.PK 113-7D (MATa)
    • Sample_biomaterial_provider_ch1: E Suir
    • Sample_treatment_protocol_ch1: liquid N2 quenching
    • Sample_growth_protocol_ch1: The laboratory reference strain CEN.PK 113-7D (MATa) was grown at 30 °C in 2-L chemostat fermentors (Applikon, Schiedam, The Netherlands) with a working volume of 1-L using an electronic level sensor to maintain a constant volume. All cultures, including the reference, were fed with minimal medium as described by Verduyn et al. (1992) with 25 g L-1 galactose as the limiting nutrient and 0.15 ml L-1 silicone antifoam (BDH, Poole, England) to prevent excessive foaming. The dilution rate was set to 0.10 h-1 and the pH was controlled at 5.0 with the automatic addition (ADI 1031 bio controller, Applikon) of 2 M KOH. The stirrer speed was set at 800 RPM and aerotion was maintained by sparging the fermentor with air gas at 500 ml min-1. Verduyn C, Postma E, Scheffers WA & van Dijken JP (1992) Effect of benzoic acid on metabolic fluxes in yeast: a continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast 8: 501-517.
    • Sample_molecule_ch1: polyA RNA
    • Sample_extract_protocol_ch1: Sampling of chemostat cultures and probe preparation was performed as described previously (Piper et al., 2002).
    • Sample_extract_protocol_ch1: Piper MDW, Daran-Lapujade P, Bro C, Regenberg B, Knudsen S, Nielsen J & Pronk JT (2002) Reproducibility of oligonucleotide microarray transcriptome analyses. An interlaboratory comparison using chemostat cultures of Saccharomyces cerevisiae. J Biol Chem 277: 37001-37008.
    • Sample_label_ch1: SAPE
    • Sample_label_protocol_ch1: Sampling of chemostat cultures, probe preparation and hybridization to Affymetrix GeneChip microarrays was performed as described previously (Piper et al., 2002)
    • Sample_label_protocol_ch1: Piper MDW, Daran-Lapujade P, Bro C, Regenberg B, Knudsen S, Nielsen J & Pronk JT (2002) Reproducibility of oligonucleotide microarray transcriptome analyses. An interlaboratory comparison using chemostat cultures of Saccharomyces cerevisiae. J Biol Chem 277: 37001-37008.
    • Sample_hyb_protocol: According to manufacturer's procedures
    • Sample_scan_protocol: Data acquisition was performed using the Affymetrix scanner 3000, quantification of array images and data filtering were performed with the Affymetrix software packages GCOS 1.2
    • Sample_description: ES02
    • Sample_data_processing: Chip file targetted to 150 GCOS (Affymetrix - version 1.2.0.037)
    • Sample_platform_id: GPL90
    • Sample_contact_name: Jean-Marc,,Daran
    • Sample_contact_email: j.g.daran@tudelft.nl
    • Sample_contact_phone: +31 15 278 2412
    • Sample_contact_fax: +31 15 278 23 55
    • Sample_contact_laboratory: Kluyver centre for genomics of industrial organisms
    • Sample_contact_department: Department of Biotechnology
    • Sample_contact_institute: Delft University of Technology
    • Sample_contact_address: Julianalaan 67
    • Sample_contact_city: Delft
    • Sample_contact_zip/postal_code: 2628BC
    • Sample_contact_country: Netherlands
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM283nnn/GSM283695/GSM283695.CEL.gz
    • Sample_series_id: GSE11452
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Aerobic galactose C-lim chemostat culture-2
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 0.1 0.6 A
    AFFX-MurIL10_at 0.2 0.3 A
    AFFX-MurIL4_at 0.5 0.2 A
    AFFX-MurFAS_at 1.3 0.5 A
    AFFX-BioB-5_at 97.5 52.9 P
    AFFX-BioB-M_at 122 81 P
    AFFX-BioB-3_at 117 76.1 P
    AFFX-BioC-5_at 157.7 115.8 P
    AFFX-BioC-3_at 133.2 91.2 P
    AFFX-BioDn-5_at 166.1 122.7 P
    View Full Table






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