1 sample(s) found by the keyword GSM253529.


  1. GEO sample ID: GSM253529
    • Sample_geo_accession: GSM253529
    • Sample_status: Public on May 29 2009
    • Sample_submission_date: Dec 27 2007
    • Sample_last_update_date: May 29 2009
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: isogenic Δrsf2 mutant yeast grown to A600=0.6 in YPG
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: isogenic Δrsf2 mutant
    • Sample_treatment_protocol_ch1: Cells grown to A600
    • Sample_growth_protocol_ch1: Cells grown to A600
    • Sample_molecule_ch1: polyA RNA
    • Sample_extract_protocol_ch1: total nucleic acids were extracted using the "hot phenol extraction" method (McEntee and Hudson, Anal Biochem. 1989 176(2):303-6.) PolyA mRNA was prepared according to oligotex manual specifications.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: 1 µg ds cDNA was labeled according to Affymetrix manual specifications.
    • Sample_hyb_protocol: Following fragmentation, 15 µg of cRNA were hybridized for 16 hours at 37ºC. Fragmentation and hybridization were performed at the MSU Research Tecnhology Support
    • Sample_scan_protocol: Gene Chips were scanned in a high density GC3000 scanner using GCOS operating software
    • Sample_description: PMID: 16208474
    • Sample_description: Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S cerevisiae (submitted)
    • Sample_data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0). Median of each chip scaled to 500, values less than 0.01 set to 0.01. MSU Research Tecnhology
    • Sample_platform_id: GPL90
    • Sample_contact_name: George,Gordon,Roberts III
    • Sample_contact_laboratory: Hudson
    • Sample_contact_department: Immunology/Microbiology
    • Sample_contact_institute: Wayne State University
    • Sample_contact_address: 540 E. Canfield
    • Sample_contact_city: Detroit
    • Sample_contact_state: MI
    • Sample_contact_zip/postal_code: 48201
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM253nnn/GSM253529/GSM253529.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM253nnn/GSM253529/GSM253529.chp.gz
    • Sample_series_id: GSE10031
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: WT strain Euroscarf Y10000 R1
    ID_REF Corrected Value VALUE ABS_CALL
    10000_at 6.4 15.6 P
    10001_at 250.9 255.3 P
    10002_i_at 2371.8 2353.8 A
    10003_f_at 4110.8 4091.9 P
    10004_at 1.3 0.5 A
    10005_at 149.7 215.5 P
    10006_at 21.7 133.2 P
    10007_at 240.2 324.6 P
    10008_at 0.2 3.5 A
    10009_at 0.4 3 A
    View Full Table






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