1 sample(s) found by the keyword GSM253525.


  1. GEO sample ID: GSM253525
    • Sample_geo_accession: GSM253525
    • Sample_status: Public on May 29 2009
    • Sample_submission_date: Dec 27 2007
    • Sample_last_update_date: May 29 2009
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: isogenic Δrsf1 mutant yeast grown to A600=0.6 in YPG
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: isogenic Δrsf1 mutant
    • Sample_treatment_protocol_ch1: Cells grown to A600
    • Sample_growth_protocol_ch1: Cells grown to A600
    • Sample_molecule_ch1: polyA RNA
    • Sample_extract_protocol_ch1: total nucleic acids were extracted using the "hot phenol extraction" method (McEntee and Hudson, Anal Biochem. 1989 176(2):303-6.) PolyA mRNA was prepared according to oligotex manual specifications.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: 1 µg ds cDNA was labeled according to Affymetrix manual specifications.
    • Sample_hyb_protocol: Following fragmentation, 15 µg of cRNA were hybridized for 16 hours at 37ºC. Fragmentation and hybridization were performed at the MSU Research Technology Support Facility
    • Sample_scan_protocol: Gene Chips were scanned in a high density GC3000 scanner using GCOS operating software
    • Sample_description: Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S cerevisiae (submitted)
    • Sample_data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0). Median of each chip scaled to 500, values less than 0.01 set to 0.01 at the MSU Research Technology Support Facility
    • Sample_platform_id: GPL90
    • Sample_contact_name: George,Gordon,Roberts III
    • Sample_contact_laboratory: Hudson
    • Sample_contact_department: Immunology/Microbiology
    • Sample_contact_institute: Wayne State University
    • Sample_contact_address: 540 E. Canfield
    • Sample_contact_city: Detroit
    • Sample_contact_state: MI
    • Sample_contact_zip/postal_code: 48201
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM253nnn/GSM253525/GSM253525.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM253nnn/GSM253525/GSM253525.CHP.gz
    • Sample_series_id: GSE10031
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Δrsf1 YPG biological-rep3
    ID_REF Corrected Value VALUE ABS_CALL
    10000_at 19.9 22.2 A
    10001_at 221.4 206.6 P
    10002_i_at 1868.7 1847.2 A
    10003_f_at 5333.9 5294.8 P
    10004_at 4.2 2.6 A
    10005_at 216.9 250.2 P
    10006_at 13.4 83.1 P
    10007_at 147.2 230.3 P
    10008_at 3 47.3 A
    10009_at 22.4 99.1 A
    View Full Table






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