1 sample(s) found by the keyword GSM253519.


  1. GEO sample ID: GSM253519
    • Sample_geo_accession: GSM253519
    • Sample_status: Public on May 29 2009
    • Sample_submission_date: Dec 27 2007
    • Sample_last_update_date: May 29 2009
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: isogenic Δrsf1 mutant yeast grown to A600=0.6 in YPD then switched to YPG for 15 minutes
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: isogenic Δrsf1 mutant
    • Sample_treatment_protocol_ch1: Cells grown to A600
    • Sample_growth_protocol_ch1: Cells grown to A600
    • Sample_molecule_ch1: polyA RNA
    • Sample_extract_protocol_ch1: total nucleic acids were extracted using the "hot phenol extraction" method (McEntee and Hudson, Anal Biochem. 1989 176(2):303-6.) PolyA mRNA was prepared according to oligotex manual specifications.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: 1 µg ds cDNA was labeled according to Affymetrix manual specifications.
    • Sample_hyb_protocol: Following fragmentation, 15 µg of cRNA were hybridized for 16 hours at 37ºC. Fragmentation and hybridization were performed at the MSU Research Tecnhology Support
    • Sample_scan_protocol: Gene Chips were scanned in a high density GC3000 scanner using GCOS operating software
    • Sample_description: Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S cerevisiae (submitted)
    • Sample_data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0). Median of each chip scaled to 500, values less than 0.01 set to 0.01. MSU Research Tecnhology
    • Sample_platform_id: GPL90
    • Sample_contact_name: George,Gordon,Roberts III
    • Sample_contact_laboratory: Hudson
    • Sample_contact_department: Immunology/Microbiology
    • Sample_contact_institute: Wayne State University
    • Sample_contact_address: 540 E. Canfield
    • Sample_contact_city: Detroit
    • Sample_contact_state: MI
    • Sample_contact_zip/postal_code: 48201
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM253nnn/GSM253519/GSM253519.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM253nnn/GSM253519/GSM253519.CHP.gz
    • Sample_series_id: GSE10031
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Δrsf1 YPG30min biological-rep1
    ID_REF Corrected Value VALUE ABS_CALL
    10000_at 31.3 9.7 A
    10001_at 233.7 202 P
    10002_i_at 1662.9 1633.2 A
    10003_f_at 6004.5 6013.4 P
    10004_at 0.9 1.5 A
    10005_at 420 502.6 P
    10006_at 62 198.6 P
    10007_at 225.4 334.4 P
    10008_at 0 3.3 A
    10009_at 0.6 34.6 A
    View Full Table






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