1 sample(s) found by the keyword GSM253518.


  1. GEO sample ID: GSM253518
    • Sample_geo_accession: GSM253518
    • Sample_status: Public on May 29 2009
    • Sample_submission_date: Dec 27 2007
    • Sample_last_update_date: May 29 2009
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: isogenic Δrsf1 mutant yeast grown to A600=0.6 in YPD
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: isogenic Δrsf1 mutant
    • Sample_treatment_protocol_ch1: Cells grown to A600
    • Sample_growth_protocol_ch1: Cells grown to A600
    • Sample_molecule_ch1: polyA RNA
    • Sample_extract_protocol_ch1: total nucleic acids were extracted using the "hot phenol extraction" method (McEntee and Hudson, Anal Biochem. 1989 176(2):303-6.) PolyA mRNA was prepared according to oligotex manual specifications.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: 1 µg ds cDNA was labeled according to Affymetrix manual specifications.
    • Sample_hyb_protocol: Following fragmentation, 15 µg of cRNA were hybridized for 16 hours at 37ºC. Fragmentation and hybridization were performed at the MSU Research Tecnhology Support
    • Sample_scan_protocol: Gene Chips were scanned in a high density GC3000 scanner using GCOS operating software
    • Sample_description: Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S cerevisiae (submitted)
    • Sample_data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0). Median of each chip scaled to 500, values less than 0.01 set to 0.01. MSU Research Tecnhology
    • Sample_platform_id: GPL90
    • Sample_contact_name: George,Gordon,Roberts III
    • Sample_contact_laboratory: Hudson
    • Sample_contact_department: Immunology/Microbiology
    • Sample_contact_institute: Wayne State University
    • Sample_contact_address: 540 E. Canfield
    • Sample_contact_city: Detroit
    • Sample_contact_state: MI
    • Sample_contact_zip/postal_code: 48201
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM253nnn/GSM253518/GSM253518.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM253nnn/GSM253518/GSM253518.CHP.gz
    • Sample_series_id: GSE10031
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Δrsf1 YPG15 biological-rep1
    ID_REF Corrected Value VALUE ABS_CALL
    10000_at 23.9 17.2 A
    10001_at 340.7 314 P
    10002_i_at 3147.8 3112.9 A
    10003_f_at 8740.1 8700.6 P
    10004_at 2.2 1.3 A
    10005_at 285.4 369.4 P
    10006_at 7.2 112.4 P
    10007_at 43.8 200.5 P
    10008_at 0.4 8.7 A
    10009_at 2 35.2 A
    View Full Table






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