1 sample(s) found by the keyword GSM253510.


  1. GEO sample ID: GSM253510
    • Sample_geo_accession: GSM253510
    • Sample_status: Public on May 29 2009
    • Sample_submission_date: Dec 27 2007
    • Sample_last_update_date: May 29 2009
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: wild type yeast grown to A600=0.6 in YPD then switched to YPG for 30 minutes
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: wild type parent BY4741
    • Sample_treatment_protocol_ch1: Cells grown to A600
    • Sample_growth_protocol_ch1: Cells grown to A600
    • Sample_molecule_ch1: polyA RNA
    • Sample_extract_protocol_ch1: total nucleic acids were extracted using the "hot phenol extraction" method (McEntee and Hudson, Anal Biochem. 1989 176(2):303-6.) PolyA mRNA was prepared according to oligotex manual specifications.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: 1 µg ds cDNA was labeled according to Affymetrix manual specifications.
    • Sample_hyb_protocol: Following fragmentation, 15 µg of cRNA were hybridized for 16 hours at 37ºC. Fragmentation and hybridization were performed at the MSU Research Tecnhology Support
    • Sample_scan_protocol: Gene Chips were scanned in a high density GC3000 scanner using GCOS operating software
    • Sample_description: PMID: 16741729
    • Sample_description: Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S cerevisiae (submitted)
    • Sample_data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0). Median of each chip scaled to 500, values less than 0.01 set to 0.01. MSU Research Tecnhology
    • Sample_platform_id: GPL90
    • Sample_contact_name: George,Gordon,Roberts III
    • Sample_contact_laboratory: Hudson
    • Sample_contact_department: Immunology/Microbiology
    • Sample_contact_institute: Wayne State University
    • Sample_contact_address: 540 E. Canfield
    • Sample_contact_city: Detroit
    • Sample_contact_state: MI
    • Sample_contact_zip/postal_code: 48201
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM253nnn/GSM253510/GSM253510.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM253nnn/GSM253510/GSM253510.CHP.gz
    • Sample_series_id: GSE10031
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: BY4741 YPG30min biological-rep2
    ID_REF Corrected Value VALUE ABS_CALL
    10000_at 15.5 11.2 P
    10001_at 341.2 327.1 P
    10002_i_at 1023.2 1008.3 A
    10003_f_at 3422.2 3420.5 P
    10004_at 1.4 0.5 A
    10005_at 350.8 390.7 P
    10006_at 17.7 91.7 P
    10007_at 262.6 332.2 P
    10008_at 0.1 3.9 A
    10009_at 4.1 47.7 A
    View Full Table






Developed by Dirar Homouz, Gang Chen, and Andrzej S. Kudlicki*

Software download