1 sample(s) found by the keyword GSM240647.


  1. GEO sample ID: GSM240647
    • Sample_geo_accession: GSM240647
    • Sample_status: Public on Nov 01 2007
    • Sample_submission_date: Oct 31 2007
    • Sample_last_update_date: Oct 31 2007
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: HFY1400
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Genotype, nmd2::HIS3
    • Sample_growth_protocol_ch1: Cells were grown in SC medium containing galatocse but lacking uracile at 30°C
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Hot phenol
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
    • Sample_hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast YG-S98 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
    • Sample_scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
    • Sample_description: a nmd2::HIS3 strain harboring an empty URA3 vector
    • Sample_data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Feng,,He
    • Sample_contact_email: feng.he@umassmed.edu
    • Sample_contact_phone: 508-856-3931
    • Sample_contact_fax: 508-856-5920
    • Sample_contact_department: Molecular Genetics and Microbiology
    • Sample_contact_institute: University of Massachusetts Medical School
    • Sample_contact_address: 55 Lake Avenue North
    • Sample_contact_city: Worcester
    • Sample_contact_state: MA
    • Sample_contact_zip/postal_code: 01655
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM240nnn/GSM240647/GSM240647.CEL.gz
    • Sample_series_id: GSE9482
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: A-nmd2D-time 20 min, biological replicate 1
    ID_REF Corrected Value VALUE ABS_CALL
    10000_at 3.2 3.1 A
    10001_at 331.8 328.7 P
    10002_i_at 1175.7 1173.9 A
    10003_f_at 2505.7 2496.9 P
    10004_at 1.3 2.4 A
    10005_at 98.7 123.9 P
    10006_at 2.2 34.3 P
    10007_at 660.7 691.5 P
    10008_at 0.2 2.4 A
    10009_at 1.9 15.1 A
    View Full Table






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