1 sample(s) found by the keyword GSM216552.


  1. GEO sample ID: GSM216552
    • Sample_geo_accession: GSM216552
    • Sample_status: Public on Sep 07 2007
    • Sample_submission_date: Aug 09 2007
    • Sample_last_update_date: Sep 07 2007
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: S.cerevisiae after 60min on LiCl treatment
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: S.cerevisiae after 60min on LiCl treatment
    • Sample_treatment_protocol_ch1: After incubation of the resultant transformants in SC-Ura or Sc-Leu-Ura medium at 25°C to the exponential growth phase, 200 mM LiCl was added. At the intervals (0-60 min), the cells were harvested and lysed.
    • Sample_growth_protocol_ch1: The media used for S. cerevisiae were SC consisting of 2% glucose, 0.67% Bacto Yeast Nitrogen Base with ammonium sulfate (Difco Laboratories, Detroit, MI) and drop-out mix lacking leucine (SC-Leu), or lacking leucine and uracil (SC-Leu-Ura).
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Total RNA from S. cerevisiae was isolated by the method of Köhrer and Domdey. Poly A mRNA was enriched from total RNA by Oligotex dT30 mRNA purification kit (Takara Bio).
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Accoding to the Affymetrix user's mannual
    • Sample_hyb_protocol: The biotinyated cRNA (15 µg) prob was hybridized to DNA microarray at 45°C for 18 h
    • Sample_scan_protocol: The scanning of arrays was carried out using the GeneArray scanner (Agilent technologies) Palto Alto, CA).
    • Sample_description: POG1 overexpression 60min
    • Sample_data_processing: The fluorescence intensities of array images were quantified using the Affymetrix software package (Microarray Suite version 4.0.1). The statistical analysis after data acquisition and normalization of expression data were carried out using the GeneSpring
    • Sample_platform_id: GPL90
    • Sample_contact_name: Yoshinori,,Murata
    • Sample_contact_email: muratayo@affrc.go.jp
    • Sample_contact_phone: +81-29-838-6623
    • Sample_contact_fax: +81-29-838-6623
    • Sample_contact_department: Post-harvest Science and Technology Division
    • Sample_contact_institute: Japan International Research Center for Agricultural Science
    • Sample_contact_address: 1-1 Ohwashi
    • Sample_contact_city: Tsukuba
    • Sample_contact_state: Ibaraki
    • Sample_contact_zip/postal_code: 305-8686
    • Sample_contact_country: Japan
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM216nnn/GSM216552/GSM216552.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM216nnn/GSM216552/GSM216552.CHP.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM216nnn/GSM216552/GSM216552_Normalized_POG1_60min.txt.gz
    • Sample_series_id: GSE8729
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Wild-type for H2A^4-20 and H2A K4,7G replicate 1
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 1.76251 13.1675 A
    AFFX-MurIL10_at 1.77749 1.73847 A
    AFFX-MurIL4_at 5.59432 1.61189 A
    AFFX-MurFAS_at 30.4741 20.783 A
    AFFX-BioB-5_at 205.911 160.864 P
    AFFX-BioB-M_at 281.655 240.887 P
    AFFX-BioB-3_at 211.881 177.366 P
    AFFX-BioC-5_at 457.076 420.107 P
    AFFX-BioC-3_at 426.941 388.019 P
    AFFX-BioDn-5_at 797.317 758.227 P
    View Full Table






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