1 sample(s) found by the keyword GSM216549.


  1. GEO sample ID: GSM216549
    • Sample_geo_accession: GSM216549
    • Sample_status: Public on Sep 07 2007
    • Sample_submission_date: Aug 09 2007
    • Sample_last_update_date: Sep 07 2007
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: S.cerevisiae before LiCl treatment
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: S.cerevisiae before LiCl treatment
    • Sample_treatment_protocol_ch1: After incubation of the resultant transformants in SC-Ura or Sc-Leu-Ura medium at 25°C to the exponential growth phase, 200 mM LiCl was added. At the intervals (0-60 min), the cells were harvested and lysed.
    • Sample_growth_protocol_ch1: The media used for S. cerevisiae were SC consisting of 2% glucose, 0.67% Bacto Yeast Nitrogen Base with ammonium sulfate (Difco Laboratories, Detroit, MI) and drop-out mix lacking leucine (SC-Leu), or lacking leucine and uracil (SC-Leu-Ura).
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Total RNA from S. cerevisiae was isolated by the method of Köhrer and Domdey. Poly A mRNA was enriched from total RNA by Oligotex dT30 mRNA purification kit (Takara Bio).
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Accoding to the Affymetrix user's mannual
    • Sample_hyb_protocol: The biotinyated cRNA (15 µg) prob was hybridized to DNA microarray at 45°C for 18 h
    • Sample_scan_protocol: The scanning of arrays was carried out using the GeneArray scanner (Agilent technologies) Palto Alto, CA).
    • Sample_description: KIN28 overexpression 0min
    • Sample_data_processing: The fluorescence intensities of array images were quantified using the Affymetrix software package (Microarray Suite version 4.0.1). The statistical analysis after data acquisition and normalization of expression data were carried out using the GeneSpring
    • Sample_platform_id: GPL90
    • Sample_contact_name: Yoshinori,,Murata
    • Sample_contact_email: muratayo@affrc.go.jp
    • Sample_contact_phone: +81-29-838-6623
    • Sample_contact_fax: +81-29-838-6623
    • Sample_contact_department: Post-harvest Science and Technology Division
    • Sample_contact_institute: Japan International Research Center for Agricultural Science
    • Sample_contact_address: 1-1 Ohwashi
    • Sample_contact_city: Tsukuba
    • Sample_contact_state: Ibaraki
    • Sample_contact_zip/postal_code: 305-8686
    • Sample_contact_country: Japan
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM216nnn/GSM216549/GSM216549.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM216nnn/GSM216549/GSM216549.CHP.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM216nnn/GSM216549/GSM216549_Normalized_KIN28_0min.txt.gz
    • Sample_series_id: GSE8729
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: KIN28 60min
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 0.631932 2.845 A
    AFFX-MurIL10_at 1.01651 1.38058 A
    AFFX-MurIL4_at 2.13002 1.76878 A
    AFFX-MurFAS_at 3.58227 1.45315 A
    AFFX-BioB-5_at 182.514 139.495 P
    AFFX-BioB-M_at 198.127 162.712 P
    AFFX-BioB-3_at 183.946 152.087 P
    AFFX-BioC-5_at 269.837 236.74 P
    AFFX-BioC-3_at 286.645 246.676 P
    AFFX-BioDn-5_at 463.171 427.101 P
    View Full Table






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