1 sample(s) found by the keyword GSM215121.

  1. GEO sample ID: GSM215121
    • Sample_geo_accession: GSM215121
    • Sample_status: Public on Jul 31 2009
    • Sample_submission_date: Aug 03 2007
    • Sample_last_update_date: Jul 24 2008
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: Baker’s yeast, freeze, 7day
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Commercial products of compressed yeast for bread making
    • Sample_treatment_protocol_ch1: The compressed yeast was suspended in liquid fermentation (LS) without sucrose and maltose LF-S medium (10 ml) at the concentration of OD600=30, and the suspension was frozen at -20oC for 7 day. The frozen samples were thawed at 30oC for 10 min in a water bath.
    • Sample_molecule_ch1: polyA RNA
    • Sample_extract_protocol_ch1: RNA was extracted from the yeast cells using the hot phenol method. Poly(A)+ RNA was enriched from total RNA by using an Oligotex dT30 (Super) mRNA purification kit (Takara Bio, Ohtsu, Japan).
    • Sample_label_ch1: Biotin
    • Sample_label_protocol_ch1: cDNA synthesis, cRNA synthesis, and labeling were performed according to the Affymetrix user’s manual (Affymetrix, Santa Clara, USA).
    • Sample_hyb_protocol: Affymetrix Yeast Genome S98 arrays were used as a DNA microarray. The probe was hybridized to a DNA microarray at 45oC for 18 h according to the Affymetrix user’s manual (Affymetrix, Santa Clara, USA).
    • Sample_scan_protocol: The washing and staining of arrays were performed using a GeneChip Fluidics Station 400. The scanning of arrays was carried out using a GeneChip scanner (Affymetrix).
    • Sample_description: We loaded freeze injuries into baker's yeast cells under different freezing period and analyzed gene expressions during recovery process from freeze injured stated.
    • Sample_data_processing: The fluorescence intensities of array images were quantified using the Affymetrix software package (GeneChip Operating Software 1.2). Statistical analysis after data acquisition and normalization of expression data were carried out using GeneSpring ver.7 (Agilent technologies, Palo Alto, USA). After data transformation (set measurements less than 0.01 to 0.01) to GeneSpring, per chip normalization to the 50th percentile was performed. Per gene normalization to control samples (freezing time was 0day) was applied to the per chip normalized values.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Satomi,Mizukami,Murata
    • Sample_contact_email: samymm@affrc.go.jp
    • Sample_contact_phone: +81-298-838-8066
    • Sample_contact_fax: +81-298-838-8066
    • Sample_contact_laboratory: Yeast
    • Sample_contact_institute: National Food Research Institute
    • Sample_contact_address: Kannondai 2-1-12
    • Sample_contact_city: Tsukuba
    • Sample_contact_zip/postal_code: 305-8642
    • Sample_contact_country: Japan
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM215nnn/GSM215121/GSM215121.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM215nnn/GSM215121/GSM215121.CHP.gz
    • Sample_series_id: GSE8673
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Baker's yeast_freeze_14day_rep1
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 0.641934 15.0656 A
    AFFX-MurIL10_at 0.954533 3.99779 A
    AFFX-MurIL4_at 7.44356 25.996 A
    AFFX-MurFAS_at 11.2926 5.72915 A
    AFFX-BioB-5_at 162.197 71.0104 P
    AFFX-BioB-M_at 147.565 62.2303 P
    AFFX-BioB-3_at 150.013 67.3578 P
    AFFX-BioC-5_at 246.825 157.706 P
    AFFX-BioC-3_at 260.207 172.753 P
    AFFX-BioDn-5_at 296.66 202.433 P
    View Full Table

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