1 sample(s) found by the keyword GSM206392.


  1. GEO sample ID: GSM206392
    • Sample_geo_accession: GSM206392
    • Sample_status: Public on Jun 29 2007
    • Sample_submission_date: Jun 28 2007
    • Sample_last_update_date: Jun 28 2007
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: Yeast with deletion of endogenous Pdr1 gene and expressing Pdr1-Cyc8 fusion protein
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Yeast strain BY4741
    • Sample_growth_protocol_ch1: Yeast were grown in synthetic dextrose media, logarithmically growing, 30 degrees centigrade
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Extraction of total RNA using Ambion RiboPure-Yeast protocol according to the manufacturer's instructions.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, revision 4, Affymetrix).
    • Sample_hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast Genome Array YG-s98. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
    • Sample_scan_protocol: GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
    • Sample_description: Gene expression data from yeast containing deletion of endogenous Pdr1 gene and expressing Pdr1-Cyc8 fusion protein
    • Sample_data_processing: The data were analyzed with GeneChip Operating Software version 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Geoffrey,,Neale
    • Sample_contact_email: geoffrey.neale@stjude.org
    • Sample_contact_institute: St Jude Childrens Research Hospital
    • Sample_contact_address: 332 N Lauderdale St
    • Sample_contact_city: Memphis
    • Sample_contact_state: TN
    • Sample_contact_zip/postal_code: 38105
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM206nnn/GSM206392/GSM206392.CEL.gz
    • Sample_series_id: GSE8326
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Pdr1-Sin3 fusion, biological rep1
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 2.5 12.2 A
    AFFX-MurIL10_at 15.2 1.7 A
    AFFX-MurIL4_at 47.6 2.3 A
    AFFX-MurFAS_at 75.1 1.9 A
    AFFX-BioB-5_at 533.9 358.5 P
    AFFX-BioB-M_at 438.3 279.2 P
    AFFX-BioB-3_at 400.5 252.2 P
    AFFX-BioC-5_at 412 258.2 P
    AFFX-BioC-3_at 352.4 195 P
    AFFX-BioDn-5_at 1018.4 853.3 P
    View Full Table






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