1 sample(s) found by the keyword GSM206391.


  1. GEO sample ID: GSM206391
    • Sample_geo_accession: GSM206391
    • Sample_status: Public on Jun 29 2007
    • Sample_submission_date: Jun 28 2007
    • Sample_last_update_date: Jun 28 2007
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: Yeast with deletion of endogenous Pdr1 gene and expressing Pdr1-Cyc8 fusion protein
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Yeast strain BY4741
    • Sample_growth_protocol_ch1: Yeast were grown in synthetic dextrose media, logarithmically growing, 30 degrees centigrade
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Extraction of total RNA using Ambion RiboPure-Yeast protocol according to the manufacturer's instructions.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, revision 4, Affymetrix).
    • Sample_hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast Genome Array YG-s98. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
    • Sample_scan_protocol: GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
    • Sample_description: Gene expression data from yeast containing deletion of endogenous Pdr1 gene and expressing Pdr1-Cyc8 fusion protein
    • Sample_data_processing: The data were analyzed with GeneChip Operating Software version 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Geoffrey,,Neale
    • Sample_contact_email: geoffrey.neale@stjude.org
    • Sample_contact_institute: St Jude Childrens Research Hospital
    • Sample_contact_address: 332 N Lauderdale St
    • Sample_contact_city: Memphis
    • Sample_contact_state: TN
    • Sample_contact_zip/postal_code: 38105
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM206nnn/GSM206391/GSM206391.CEL.gz
    • Sample_series_id: GSE8326
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Pdr1-Cyc8 fusion Dpdr1, biological rep3
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 0.5 37.6 A
    AFFX-MurIL10_at 1.2 6.1 A
    AFFX-MurIL4_at 8.2 3.3 A
    AFFX-MurFAS_at 15.7 5.2 A
    AFFX-BioB-5_at 475.9 317.4 P
    AFFX-BioB-M_at 730.2 595.9 P
    AFFX-BioB-3_at 518.7 391 P
    AFFX-BioC-5_at 494.9 362.4 P
    AFFX-BioC-3_at 489.7 350.1 P
    AFFX-BioDn-5_at 1635.9 1494.5 P
    View Full Table






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