1 sample(s) found by the keyword GSM184974.


  1. GEO sample ID: GSM184974
    • Sample_geo_accession: GSM184974
    • Sample_status: Public on Oct 24 2007
    • Sample_submission_date: Apr 26 2007
    • Sample_last_update_date: Jul 10 2007
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: yeast, CHP, t=70 min, replicate 1
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Strain BY4743
    • Sample_characteristics_ch1: Mid-exponental phase
    • Sample_treatment_protocol_ch1: In mid-exponential phase (OD600 ~ 1.5), three replicate cultures were treated with 0.19 mM CHP, while three untreated cultures were used as controls.
    • Sample_growth_protocol_ch1: Yeast cultures were grown in controlled batch conditions, in 1 L fermentors at 30C, pH 6.0, 500 rpm, and dO2> 80%.
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: RNA was extracted with a procedure modified from the hot phenol protocol, as described (Martins, A. et al (2007) Yeast 24, 181-188)
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
    • Sample_hyb_protocol: Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast S98 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 640.
    • Sample_scan_protocol: GeneChips were scanned using the Affymetrix 7G Scanner.
    • Sample_description: Culture treated with CHP 0.19 mM, collected at t=70 min (after adding CHP), biological replicate 1
    • Sample_data_processing: The data were normalized with RMA.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Vladimir,,Shulaev
    • Sample_contact_email: vshulaev@vbi.vt.edu
    • Sample_contact_institute: Virginia Bioinformatics Institute
    • Sample_contact_address: Virginia Bioinformatics Inst., Virginia Tech, Washington st.
    • Sample_contact_city: Blacksburg
    • Sample_contact_state: VA
    • Sample_contact_zip/postal_code: 24061
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM184nnn/GSM184974/GSM184974.CEL.gz
    • Sample_series_id: GSE7645
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: CHP, t=120 min, biological replicate 1
    ID_REF Corrected Value VALUE
    10000_at 4.400952577 3.165788331
    10001_at 8.571050505 8.459179371
    10002_i_at 9.582938696 9.518127119
    10003_f_at 11.63480746 11.62377101
    10004_at 3.308425696 3.273483539
    10005_at 7.015046625 7.342125726
    10006_at 3.206025087 6.012019231
    10007_at 9.660896292 9.79101039
    10008_at 2.531651094 5.454039659
    10009_at 2.537345026 5.144176935
    View Full Table






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