1 sample(s) found by the keyword GSM153631.


  1. GEO sample ID: GSM153631
    • Sample_geo_accession: GSM153631
    • Sample_status: Public on Apr 10 2007
    • Sample_submission_date: Dec 29 2006
    • Sample_last_update_date: Dec 29 2006
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: 0 hr after transfer into sporulation medium
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: strain: RKD101
    • Sample_characteristics_ch1: background: SK1
    • Sample_characteristics_ch1: genotype: rad50delta
    • Sample_treatment_protocol_ch1: Cells were washed twice with water, frozen in liquid nitrogen, and stored at -80C.
    • Sample_growth_protocol_ch1: Cells were grown on a YPD plate for 2 days at 30C. The cells were grown to a density of 4.0x10^7 cells/ml at 30C in SPS presporulation medium (0.5% yeast extract, 1% peptone, 0.17% yeast nitrogen base w/o amino acid and ammonium sulfate, 1% potassium acetate, 0.5% ammonium sulfate, usual level fo amino acids, 50 mM potassium phthalate buffer pH 5.0, and antifoam).
    • Sample_molecule_ch1: polyA RNA
    • Sample_extract_protocol_ch1: Total RNA was prepared using glass beads and RNeasy Maxi Kit (QIAGEN). mRNA was isolated the total RNA using Oligotex-dT30 (TaKaRa). These processes were performed according to the manufacturer's instructions.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 microg mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
    • Sample_hyb_protocol: Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip YG-S98. GeneChips were washed and stained using EukGE-WS1 ver3 protocol in the Affymetrix Fluidics Station 400.
    • Sample_scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
    • Sample_description: Gene expression data from diploid cells in premeiosis.
    • Sample_data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Kazuto,,Kugou
    • Sample_contact_email: kkugou@riken.jp
    • Sample_contact_laboratory: Shibata Distinguished Scientist Laboratory
    • Sample_contact_institute: RIKEN
    • Sample_contact_address: 2-1 Hirosawa
    • Sample_contact_city: Wako
    • Sample_contact_state: Saitama
    • Sample_contact_zip/postal_code: 351-0198
    • Sample_contact_country: Japan
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM153nnn/GSM153631/GSM153631.CEL.gz
    • Sample_series_id: GSE6620
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Expression in rad50delta strain at meiosis 4 hr
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 0.5 6.6 A
    AFFX-MurIL10_at 0.9 3 A
    AFFX-MurIL4_at 1.5 1 A
    AFFX-MurFAS_at 2.5 2.7 A
    AFFX-BioB-5_at 239.3 139.8 P
    AFFX-BioB-M_at 399.2 309.2 P
    AFFX-BioB-3_at 351.2 269.5 P
    AFFX-BioC-5_at 469.9 384.8 P
    AFFX-BioC-3_at 466.8 382 P
    AFFX-BioDn-5_at 562.6 481.7 P
    View Full Table






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