1 sample(s) found by the keyword GSM142987.


  1. GEO sample ID: GSM142987
    • Sample_geo_accession: GSM142987
    • Sample_status: Public on Jan 22 2007
    • Sample_submission_date: Oct 27 2006
    • Sample_last_update_date: Mar 13 2008
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: Saccharomyces cerevisiae cells
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Saccharomyces cerevisiae, wt, maintianed on YPD plates, started from 48 hrs growth on a new YPD agar plate
    • Sample_treatment_protocol_ch1: Cells were grown to O.D. of 1.0, given 250?g/ml pyocyanin in minimal ethanol, and grown for 6 hours, then collected by centrifugation, frozen in liquid nitrogen, thawed before proecessing.
    • Sample_growth_protocol_ch1: Cells were grown in YPD media at 30 degrees Celsius at 250 rpm shaker.
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: SDS/double phenol RNA extraction of total RNA was performed according to the manufacturer's instructions.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
    • Sample_hyb_protocol: Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip YeastS98 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
    • Sample_scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
    • Sample_description: We have examined the transcriptional response of S. cerevisiae to exposure to pyocyanin at 25 and 250 ?g/ml concentrations. Our analysis indicates that the changes are consistent with an oxidative stress response, as observed in other studies of the mode of action of pyocyanin toxicity.
    • Sample_data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Coran,MH,Watanabe
    • Sample_contact_email: watanabe@mail.chem.tamu.edu
    • Sample_contact_phone: 979-458-8094
    • Sample_contact_fax: 979-458-8095
    • Sample_contact_laboratory: Watanabe
    • Sample_contact_department: Chemistry
    • Sample_contact_institute: Texas A*M University
    • Sample_contact_address: Texas A&M Dept. Chemistry MS 3255
    • Sample_contact_city: College Station
    • Sample_contact_state: TX
    • Sample_contact_zip/postal_code: 77843
    • Sample_contact_country: USA
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM142nnn/GSM142987/GSM142987.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM142nnn/GSM142987/GSM142987.EXP.gz
    • Sample_series_id: GSE6185
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: yeast treated with methanol, biological rep1
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 0.8 5.9 A
    AFFX-MurIL10_at 6.6 13.7 A
    AFFX-MurIL4_at 19.2 10 A
    AFFX-MurFAS_at 29.3 7.4 A
    AFFX-BioB-5_at 233.9 99.3 P
    AFFX-BioB-M_at 210.7 93.7 P
    AFFX-BioB-3_at 182.3 73.8 M
    AFFX-BioC-5_at 280.7 164.8 P
    AFFX-BioC-3_at 285.6 161.2 P
    AFFX-BioDn-5_at 191.6 65 P
    View Full Table






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