1 sample(s) found by the keyword GSM119758.


  1. GEO sample ID: GSM119758
    • Sample_geo_accession: GSM119758
    • Sample_status: Public on Jul 12 2006
    • Sample_submission_date: Jul 11 2006
    • Sample_last_update_date: Jul 11 2006
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: haploid Saccharomyces strain derived from W303a
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Genotype: strain bears two disrupted genes (hyp2 and anb1) expressing wild-type human EIF5A1(plasmid born)
    • Sample_characteristics_ch1: Growth: normal, comparable to strains expressing eIF5A protein from HYP2
    • Sample_treatment_protocol_ch1: Strains were cultured at 25°C (permissive temperature) until reaching the early lorarithmic phase of growth. 2*10^8 cells of each strain were harvested by centrifugation and briefly rinsed with 1 volume of water (4°C), cells were either frozen at -80°C or directly subjected to the isolation of total RNA.
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Hot phenol extraction of total RNA was performed according to the literature.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
    • Sample_hyb_protocol: Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast Genome Array S98. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
    • Sample_scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
    • Sample_description: Gene expression data from yeast eIF5A-delta strain expressing human wild-type EIF5A at 25°C.
    • Sample_data_processing: Scanned raw data images were processed with Affymetrix GeneChip V3.2 software. Data from the arrays was normalized (RMA), and expression values based on an additive model were calculated, according to the method of Irizarry et al. Differentially expressed genes were identified by the permutation-based method of Tusher et al.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Rainer,,Schrader
    • Sample_contact_email: schrader@biochem.mpg.de
    • Sample_contact_phone: +498985782490
    • Sample_contact_fax: +498985782802
    • Sample_contact_laboratory: Prof. Lottspeich
    • Sample_contact_department: Proteinanalytics
    • Sample_contact_institute: Max-Planck-Institut for Biochemistry
    • Sample_contact_address: Am Klopferspitz 18
    • Sample_contact_city: Martinsried
    • Sample_contact_state: Bavaria
    • Sample_contact_zip/postal_code: 82152
    • Sample_contact_country: Germany
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM119nnn/GSM119758/GSM119758.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM119nnn/GSM119758/GSM119758.EXP.gz
    • Sample_series_id: GSE5290
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Tomatine 13.9 ppm day 1
    ID_REF Corrected Value VALUE
    10000_at 194.4084 213.7026
    10001_at 2850.5012 2888.6501
    10002_i_at 21977.1916 22065.988
    10003_f_at 29202.6344 29264.4151
    10004_at 139.3622 234.3226
    10005_at 3944.2472 4327.5477
    10006_at 515.5813 1135.7762
    10007_at 5129.5988 5732.4253
    10008_at 218.0396 800.9529
    10009_at 419.6629 988.623
    View Full Table






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