1 sample(s) found by the keyword GSM119757.


  1. GEO sample ID: GSM119757
    • Sample_geo_accession: GSM119757
    • Sample_status: Public on Jul 12 2006
    • Sample_submission_date: Jul 11 2006
    • Sample_last_update_date: Jul 11 2006
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: haploid Saccharomyces strain derived from W303a
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Genotype: strain bears two disrupted genes (hyp2 and anb1) expressing wild-type human EIF5A1(plasmid born)
    • Sample_characteristics_ch1: Growth: normal, comparable to strains expressing eIF5A protein from HYP2
    • Sample_treatment_protocol_ch1: Strains were cultured at 25°C (permissive temperature) until reaching the early lorarithmic phase of growth. 2*10^8 cells of each strain were harvested by centrifugation and briefly rinsed with 1 volume of water (4°C), cells were either frozen at -80°C or directly subjected to the isolation of total RNA.
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Hot phenol extraction of total RNA was performed according to the literature.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
    • Sample_hyb_protocol: Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast Genome Array S98. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
    • Sample_scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
    • Sample_description: Gene expression data from yeast eIF5A-delta strain expressing human wild-type EIF5A at 25°C.
    • Sample_data_processing: Scanned raw data images were processed with Affymetrix GeneChip V3.2 software. Data from the arrays was normalized (RMA), and expression values based on an additive model were calculated, according to the method of Irizarry et al. Differentially expressed genes were identified by the permutation-based method of Tusher et al.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Rainer,,Schrader
    • Sample_contact_email: schrader@biochem.mpg.de
    • Sample_contact_phone: +498985782490
    • Sample_contact_fax: +498985782802
    • Sample_contact_laboratory: Prof. Lottspeich
    • Sample_contact_department: Proteinanalytics
    • Sample_contact_institute: Max-Planck-Institut for Biochemistry
    • Sample_contact_address: Am Klopferspitz 18
    • Sample_contact_city: Martinsried
    • Sample_contact_state: Bavaria
    • Sample_contact_zip/postal_code: 82152
    • Sample_contact_country: Germany
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM119nnn/GSM119757/GSM119757.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM119nnn/GSM119757/GSM119757.EXP.gz
    • Sample_series_id: GSE5290
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: wild-type control strain, biological rep 4
    ID_REF Corrected Value VALUE
    10000_at 207.5034 161.833
    10001_at 2598.1088 2613.4063
    10002_i_at 19539.6586 19617.2089
    10003_f_at 26683.773 26712.3693
    10004_at 228.761 382.0866
    10005_at 3326.47 3601.8319
    10006_at 382.546 845.6086
    10007_at 4007.1131 4474.7676
    10008_at 421.7523 928.7786
    10009_at 490.1319 934.3176
    View Full Table






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