1 sample(s) found by the keyword GSM119754.


  1. GEO sample ID: GSM119754
    • Sample_geo_accession: GSM119754
    • Sample_status: Public on Jul 12 2006
    • Sample_submission_date: Jul 11 2006
    • Sample_last_update_date: Jul 11 2006
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: haploid Saccharomyces strain derived from W303a
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Genotype: strain bears two disrupted genes (hyp2 and anb1) expressing V81G point mutated human EIF5A1(plasmid born)
    • Sample_characteristics_ch1: Growth: distinct temperature sensitivity
    • Sample_treatment_protocol_ch1: Strains were cultured at 25°C (permissive temperature) until reaching the early lorarithmic phase of growth. 2*10^8 cells of each strain were harvested by centrifugation and briefly rinsed with 1 volume of water (4°C), cells were either frozen at -80°C or directly subjected to the isolation of total RNA.
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Hot phenol extraction of total RNA was performed according to the literature.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
    • Sample_hyb_protocol: Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast Genome Array S98. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
    • Sample_scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
    • Sample_description: Gene expression data from yeast eIF5A-delta strain expressing human V81G point mutated EIF5A at 25°C.
    • Sample_data_processing: Scanned raw data images were processed with Affymetrix GeneChip V3.2 software. Data from the arrays was normalized (RMA), and expression values based on an additive model were calculated, according to the method of Irizarry et al. Differentially expressed genes were identified by the permutation-based method of Tusher et al.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Rainer,,Schrader
    • Sample_contact_email: schrader@biochem.mpg.de
    • Sample_contact_phone: +498985782490
    • Sample_contact_fax: +498985782802
    • Sample_contact_laboratory: Prof. Lottspeich
    • Sample_contact_department: Proteinanalytics
    • Sample_contact_institute: Max-Planck-Institut for Biochemistry
    • Sample_contact_address: Am Klopferspitz 18
    • Sample_contact_city: Martinsried
    • Sample_contact_state: Bavaria
    • Sample_contact_zip/postal_code: 82152
    • Sample_contact_country: Germany
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM119nnn/GSM119754/GSM119754.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM119nnn/GSM119754/GSM119754.EXP.gz
    • Sample_series_id: GSE5290
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: wild-type control strain, biological rep 1
    ID_REF Corrected Value VALUE
    10000_at 203.7143 198.1601
    10001_at 2509.951 2556.639
    10002_i_at 21353.6682 21275.388
    10003_f_at 28733.7565 28733.7565
    10004_at 261.4307 427.6252
    10005_at 3162.6915 3366.7939
    10006_at 546.2805 977.6726
    10007_at 5245.9711 5683.0453
    10008_at 400.6826 916.0277
    10009_at 427.1123 894.5803
    View Full Table






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