1 sample(s) found by the keyword GSM119753.


  1. GEO sample ID: GSM119753
    • Sample_geo_accession: GSM119753
    • Sample_status: Public on Jul 12 2006
    • Sample_submission_date: Jul 11 2006
    • Sample_last_update_date: Jul 11 2006
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: haploid Saccharomyces strain derived from W303a
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Genotype: strain bears two disrupted genes (hyp2 and anb1) expressing V81G point mutated human EIF5A1(plasmid born)
    • Sample_characteristics_ch1: Growth: distinct temperature sensitivity
    • Sample_treatment_protocol_ch1: Strains were cultured at 25°C (permissive temperature) until reaching the early lorarithmic phase of growth. 2*10^8 cells of each strain were harvested by centrifugation and briefly rinsed with 1 volume of water (4°C), cells were either frozen at -80°C or directly subjected to the isolation of total RNA.
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Hot phenol extraction of total RNA was performed according to the literature.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
    • Sample_hyb_protocol: Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast Genome Array S98. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
    • Sample_scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
    • Sample_description: Gene expression data from yeast eIF5A-delta strain expressing human V81G point mutated EIF5A at 25°C.
    • Sample_data_processing: Scanned raw data images were processed with Affymetrix GeneChip V3.2 software. Data from the arrays was normalized (RMA), and expression values based on an additive model were calculated, according to the method of Irizarry et al. Differentially expressed genes were identified by the permutation-based method of Tusher et al.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Rainer,,Schrader
    • Sample_contact_email: schrader@biochem.mpg.de
    • Sample_contact_phone: +498985782490
    • Sample_contact_fax: +498985782802
    • Sample_contact_laboratory: Prof. Lottspeich
    • Sample_contact_department: Proteinanalytics
    • Sample_contact_institute: Max-Planck-Institut for Biochemistry
    • Sample_contact_address: Am Klopferspitz 18
    • Sample_contact_city: Martinsried
    • Sample_contact_state: Bavaria
    • Sample_contact_zip/postal_code: 82152
    • Sample_contact_country: Germany
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM119nnn/GSM119753/GSM119753.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM119nnn/GSM119753/GSM119753.EXP.gz
    • Sample_series_id: GSE5290
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: mutant V81G expressing strain, biological rep4
    ID_REF Corrected Value VALUE
    10000_at 247.2737 210.9825
    10001_at 2254.5378 2262.8393
    10002_i_at 18836.0374 18905.4797
    10003_f_at 25119.9548 25050.3782
    10004_at 553.5144 808.8368
    10005_at 3902.6717 4188.6098
    10006_at 1105.951 1525.6185
    10007_at 4310.4862 4745.5884
    10008_at 358.7222 829.3102
    10009_at 433.8984 859.5145
    View Full Table






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