1 sample(s) found by the keyword GSM119752.


  1. GEO sample ID: GSM119752
    • Sample_geo_accession: GSM119752
    • Sample_status: Public on Jul 12 2006
    • Sample_submission_date: Jul 11 2006
    • Sample_last_update_date: Jul 11 2006
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: haploid Saccharomyces strain derived from W303a
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Genotype: strain bears two disrupted genes (hyp2 and anb1) expressing V81G point mutated human EIF5A1(plasmid born)
    • Sample_characteristics_ch1: Growth: distinct temperature sensitivity
    • Sample_treatment_protocol_ch1: Strains were cultured at 25°C (permissive temperature) until reaching the early lorarithmic phase of growth. 2*10^8 cells of each strain were harvested by centrifugation and briefly rinsed with 1 volume of water (4°C), cells were either frozen at -80°C or directly subjected to the isolation of total RNA.
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Hot phenol extraction of total RNA was performed according to the literature.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
    • Sample_hyb_protocol: Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast Genome Array S98. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
    • Sample_scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
    • Sample_description: Gene expression data from yeast eIF5A-delta strain expressing human V81G point mutated EIF5A at 25°C.
    • Sample_data_processing: Scanned raw data images were processed with Affymetrix GeneChip V3.2 software. Data from the arrays was normalized (RMA), and expression values based on an additive model were calculated, according to the method of Irizarry et al. Differentially expressed genes were identified by the permutation-based method of Tusher et al.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Rainer,,Schrader
    • Sample_contact_email: schrader@biochem.mpg.de
    • Sample_contact_phone: +498985782490
    • Sample_contact_fax: +498985782802
    • Sample_contact_laboratory: Prof. Lottspeich
    • Sample_contact_department: Proteinanalytics
    • Sample_contact_institute: Max-Planck-Institut for Biochemistry
    • Sample_contact_address: Am Klopferspitz 18
    • Sample_contact_city: Martinsried
    • Sample_contact_state: Bavaria
    • Sample_contact_zip/postal_code: 82152
    • Sample_contact_country: Germany
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM119nnn/GSM119752/GSM119752.CEL.gz
    • Sample_supplementary_file: ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/samples/GSM119nnn/GSM119752/GSM119752.EXP.gz
    • Sample_series_id: GSE5290
    • Sample_data_row_count: 9335
    • Sample_comment: Raw data provided as supplementary file
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: mutant V81G expressing strain, biological rep3
    ID_REF Corrected Value VALUE
    10000_at 262.37928 276.71976
    10001_at 2478.6521 2523.11327
    10002_i_at 18499.16719 18499.16719
    10003_f_at 22172.03633 22172.03633
    10004_at 267.19348 447.65895
    10005_at 2984.78781 3250.52387
    10006_at 315.17967 734.22783
    10007_at 8208.54383 8646.47096
    10008_at 538.23861 1031.63891
    10009_at 586.99921 1025.82093
    View Full Table






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