1 sample(s) found by the keyword GSM118590.


  1. GEO sample ID: GSM118590
    • Sample_geo_accession: GSM118590
    • Sample_status: Public on Nov 10 2007
    • Sample_submission_date: Jul 06 2006
    • Sample_last_update_date: Nov 09 2007
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: chemostat sampled cells
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: Strain: CEN.PK111.32D; Genotype: MATa MAL2-8c SUC2 leu2-3,112 SFP1::KlLEU2
    • Sample_molecule_ch1: total RNA
    • Sample_label_ch1: Biotin-labeled cRNA
    • Sample_description: The Saccharomyces cerevisiae sfp1 null mutant strain CEN.PK111.32D (MATa MAL2-8c SUC2 leu2-3,112 SFP1::KlLEU2) was used in this study. Cells were grown at 30°C in 2-L chemostats (Applikon), with a working volume of 1.0 L. Cultures were fed with a defined mineral medium (the medium composition was based on that described by Verduyn et al, Yeast 8:501-17, 1992) that limited growth by glucose at dilution rate of 0.05 h-1 under anaerobic conditions (maintained by sparging the medium reservoir and the fermentor with pure nitrogen gas at 0.5 l min-1). Furthermore, Norprene tubing and butyl septa were used to minimize oxygen diffusion into the anaerobic cultures. The stirrer speed was 800 rpm. The pH was measured on-line and kept constant at 5.0 by the automatic addition of 2 M KOH using an Applikon ADI 1030 Biocontroller. The working volume of the cultures was kept constant by means of an electrical level sensor. The off-gas was cooled by a condenser connected to a cryostat set at 2°C and analysed as previously described (van Maris et al, Appl. Environ. Microbiol. 69:2094-99, 1993). Chemostat cultures were assumed to be in steady-state when, after at least five volume changes, the culture dry-weight, specific carbon-dioxide production rate, changed by less than 2% during 24 hours. Steady state samples were taken from cultures below 18 generations in chemostat. Dry weight, extra-cellular metabolites, dissolved oxygen and gas profiles had to be constant over at least three volume changes prior to sampling for RNA extraction. Probe preparation and hybridization to Affymetrix Genechip® microarrays were performed following Affymetrix instructions. The one-cycle eukaryotic target labelling assay was used, starting with 15 mg total RNA. The quality of total RNA, cDNA, cRNA and fragmented cRNA were checked using the Agilent Bioanalyzer 2100 (Agilent Technologies).
    • Sample_data_processing: GeneChip? Operating Software (GCOS), version 1.2. Samples scaled to 150.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Chiara,,Cipollina
    • Sample_contact_email: c.cipollina@tudelft.nl
    • Sample_contact_department: Biotechnology
    • Sample_contact_institute: Delft University of Technology
    • Sample_contact_address: Julianalaan 67
    • Sample_contact_city: Delft
    • Sample_contact_zip/postal_code: 2628 BC
    • Sample_contact_country: Netherlands
    • Sample_supplementary_file: NONE
    • Sample_series_id: GSE5238
    • Sample_data_row_count: 9335
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: sfp1 delta Glu-lim2
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 0.3 4.7 A
    AFFX-MurIL10_at 1.3 0.6 A
    AFFX-MurIL4_at 7 4 A
    AFFX-MurFAS_at 10.7 5.2 A
    AFFX-BioB-5_at 84.6 42.3 P
    AFFX-BioB-M_at 110.9 69.1 P
    AFFX-BioB-3_at 110.2 69.9 P
    AFFX-BioC-5_at 154.6 115.7 P
    AFFX-BioC-3_at 191.6 151.8 P
    AFFX-BioDn-5_at 345.7 301 P
    View Full Table






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