1 sample(s) found by the keyword GSM105336.


  1. GEO sample ID: GSM105336
    • Sample_geo_accession: GSM105336
    • Sample_status: Public on Jul 28 2006
    • Sample_submission_date: Apr 13 2006
    • Sample_last_update_date: Jul 31 2006
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: Saccharomyces cerevisiae S288c following 5 hours exposure to 15 uM tomatidine (dissolved in the carrier solvet DMF)
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: strain: S288c
    • Sample_characteristics_ch1: Cells were pelleted and frozen in liquid nitrogen. Acid-washed glass beads (0.5 mm diameter; Sigma) were added and the cells disrupted using two 20 s cycles at speed setting 6 in the Savant Bio 101 Fast Prep FP120.
    • Sample_treatment_protocol_ch1: An overnight culture of strain S288C was diluted to an OD600nm of 0.1. After 1 hour of growth at 30?C, the compounds (or solvent alone) were added and the cultures were incubated for an additional 5 hours. Growth inhibition was monitored by measuring the OD600nm
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Total RNA was isolated using the Qiagen RNeasy kit (Qiagen, Inc., Valencia, CA, USA) was performed according to the manufacturer's instructions.
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix (Expression Analysis Technical Manual, 2001, Affymetrix & Zhu et al., 2001). ).
    • Sample_hyb_protocol: Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Yeast Genome S98 Array YG-S98. GeneChips were washed and stained in the Affymetrix Fluidics Station 400 as previously described (Zhu et al., 2001).
    • Sample_scan_protocol: GeneChips were scanned using a Affymetrix GeneChip┬« Scanner.
    • Sample_description: Gene expression data following +/- chemical treatment.
    • Sample_data_processing: Data were analyzed using the RMA algorithm using Expressionist Pro (GeneData Inc., Switzerland).
    • Sample_platform_id: GPL90
    • Sample_contact_name: Michael,,Csukai
    • Sample_contact_email: michael.csukai@syngenta.com
    • Sample_contact_institute: Syngenta
    • Sample_contact_address: Jealott's Hill
    • Sample_contact_city: Bracknell
    • Sample_contact_state: Berkshire
    • Sample_contact_zip/postal_code: RG42 6EY
    • Sample_contact_country: United Kingdom
    • Sample_supplementary_file: NONE
    • Sample_series_id: GSE4669
    • Sample_data_row_count: 9335
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: Tomatine 13.9 ppm day 2
    ID_REF Corrected Value VALUE DETECTION P-VALUE
    AFFX-MurIL2_at 4.454487 14.91306 0.98861563
    AFFX-MurIL10_at 9.287122 21.945372 0.81486934
    AFFX-MurIL4_at 10.94994 5.454818 0.9370707
    AFFX-MurFAS_at 16.744303 8.068181 0.95703804
    AFFX-BioB-5_at 117.07536 46.604317 0.15673155
    AFFX-BioB-M_at 237.85825 170.20522 1.69E-04
    AFFX-BioB-3_at 232.98598 170.41685 5.81E-04
    AFFX-BioC-5_at 342.8891 276.7702 9.45E-05
    AFFX-BioC-3_at 324.57773 253.46043 6.02E-05
    AFFX-BioDn-5_at 326.7714 254.005 4.43E-05
    View Full Table






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