1 sample(s) found by the keyword GSM105139.


  1. GEO sample ID: GSM105139
    • Sample_geo_accession: GSM105139
    • Sample_status: Public on May 30 2006
    • Sample_submission_date: Apr 11 2006
    • Sample_last_update_date: Apr 12 2006
    • Sample_type: RNA
    • Sample_channel_count: 1
    • Sample_source_name_ch1: yeast 37C 2 hours
    • Sample_organism_ch1: Saccharomyces cerevisiae
    • Sample_characteristics_ch1: strain: 1099 nab3-11
    • Sample_characteristics_ch1: media: YPD
    • Sample_characteristics_ch1: temp: 37C
    • Sample_treatment_protocol_ch1: temperature shift to 37C for 2 hours
    • Sample_growth_protocol_ch1: YPD
    • Sample_growth_protocol_ch1: temperature shift to 37C for 2 hours
    • Sample_molecule_ch1: total RNA
    • Sample_extract_protocol_ch1: Hot Acid Phenol
    • Sample_extract_protocol_ch1: RNeasy clean up kit
    • Sample_label_ch1: biotin
    • Sample_label_protocol_ch1: One round amplification protocol for total RNA following Affymetrix’ specifications using T7 promoter oligo dT primer for cDNA synthesis, and ENZO IVT kit (www.affymetrix.com, GeneChip Expression Analysis, Technical Manual, 2003).
    • Sample_hyb_protocol: Hybridization protocol: 16hrs at 45 C with rotation (60rpm) as described by Affymetrix in their GeneChip Expression Analysis Technical Manual, 2004.
    • Sample_scan_protocol: Using Affymetrix’ GeneChip Scanner 3000 and default parameters described by the manufacturer in their GeneChip Expression Analysis Technical Manual, 2004.
    • Sample_description: yeast (nab3-11) total RNA from a 2 hour temperature shift at 37C.
    • Sample_data_processing: Image analysis using GCOS 1.1.1, using MAS 5.0 algorithm and manufacturer’s specifications. Global scaling of images to a target intensity of 150.
    • Sample_platform_id: GPL90
    • Sample_contact_name: Jeff,L,Corden
    • Sample_contact_department: Molecular Biology and Genetics
    • Sample_contact_institute: Johns Hopkins School of Medicine
    • Sample_contact_address: 725 N. Wolfe St.
    • Sample_contact_city: Baltimore
    • Sample_contact_state: MD
    • Sample_contact_zip/postal_code: 21202
    • Sample_contact_country: USA
    • Sample_supplementary_file: NONE
    • Sample_series_id: GSE4657
    • Sample_data_row_count: 9335
    • sample_table_begin:
    • sample_table_end:
    • Sample_title: nab3 ts mutant at 25C
    ID_REF Corrected Value VALUE ABS_CALL
    AFFX-MurIL2_at 0 0.9 A
    AFFX-MurIL10_at 0.2 0.7 A
    AFFX-MurIL4_at 1.4 0.5 A
    AFFX-MurFAS_at 5.1 0.8 A
    AFFX-BioB-5_at 98.1 56.1 P
    AFFX-BioB-M_at 101.4 64.7 P
    AFFX-BioB-3_at 90.1 56.9 P
    AFFX-BioC-5_at 131.1 96.9 P
    AFFX-BioC-3_at 123.6 84.1 P
    AFFX-BioDn-5_at 118.3 77.8 P
    View Full Table






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